|
|
 |
Customer-tailored Antibody Services
Welcome to ARC’s services! During our services, your secrecies will be kept:
Synthesis of genetically engineered proteins
Protein expression (around 90 days)
Construction of recombined T-vector containing the target protein: Cat.No.P1
Materials provided by customers: gene sequence and the required cloning region
Acceptance standard: sequencing result
Construction of recombined expression plasmid and protein induction Cat.NO.P2
Materials provided by customers: recombined expression plasmid, sequencing result, cloning region, gene primers
Acceptance standard: SDS-PAGE
Protein purification (around a week)
Purification of proteins with His tag Cat.NO.P3 ¥3700
Purification of proteins with GST tag(including GST cleveage) Cat.NO.P4 ¥4500
Purification of proteins (by other methods) Cat.NO.P5 discuss face-to-face
Materials provided by customers: the target protein needs purifying
Acceptance standard: Purity verification picture
Polypeptide synthesis and protein conjunction
polypeptide synthesis:(10-20 working days)
polypeptide synthesis |
Cat.No.:A1 |
Prices calculated according to the list below |
|
| Amount |
Raw product |
desalted |
>75% |
>85% |
>90% |
>95% |
>98% |
| 10-14mg |
¥62 |
¥79 |
¥133 |
¥177 |
¥213 |
¥228 |
¥343 |
| 15-19mg |
¥65 |
¥81 |
¥142 |
¥197 |
¥234 |
¥255 |
¥383 |
| 20-24mg |
¥69 |
¥85 |
¥167 |
¥220 |
¥255 |
¥290 |
¥436 |
| 25-29mg |
¥74 |
¥90 |
¥182 |
¥235 |
¥270 |
¥316 |
¥473 |
| 30-39mg |
¥78 |
¥95 |
¥197 |
¥246 |
¥294 |
¥336 |
¥503 |
| 40-49mg |
¥88 |
¥105 |
¥201 |
¥260 |
¥310 |
¥357 |
¥537 |
| 50-59mg |
¥91 |
¥108 |
¥231 |
¥285 |
¥335 |
¥381 |
¥571 |
| 60-79mg |
¥108 |
¥124 |
¥270 |
¥318 |
¥381 |
¥432 |
¥649 |
| 80-100mg |
¥112 |
¥125 |
¥318 |
¥381 |
¥458 |
¥509 |
¥763 |
| 1000mg |
¥401 |
¥417 |
¥955 |
¥1142 |
¥1375 |
¥1524 |
¥2287 |
List above is the price per amino acid. Further contact is needed if your peptide is >30aa or <6aa. Large orders can enjoy a discount.
we will provide MS and HPLC report as quality verification for the peptide. Synthesis or modification of a special amino acid sequence needs further contact.
Protein conjunction (3 work days)
Protein conjunction |
Cat.No.:A2 |
¥500 |
Conjunction BSA in target peptide’s N or C terminal.
Polyclonal antibody service
Customer-tailored polyclonal antibody service |
Cat.No.:A3 |
¥3000 |
- Animal: rabbit
- Antigen provided by customers should be more than 5mg and above 90% purity, which is verified in sds-page. If the project fails, the responsibilities are on customer’s shoulders if the antigen provided cannot meet the standard.
- Immunize rabbits several times, titers are measured each time. All data will be given to the customer at the end of the project.
Provide 20ml antiserum after the last immunization. If more antiserum is needed, the price will rise proportionally. If serum before immunization is needed, notifications should be pre-made with an increased fee of 300RMB. We usually provide 1-2ml pre-immunized serum.
- Verification method: ELISA test
- Verification standard: Antigen is specially recognized by the antiserum. And we only guarantee specialized recognition of antigens provided by the customer.
- If antiserum of mice or other animals is required, the prices can be differential and further contact is needed.
- Prices listed above are confined in rabbit antiserum production.
Final provision: 20ml rabbit antiserum with a titer of more than 1:10,000. ELISA report is offered. In the process of producing and transportation, antiserum is stored in -20℃
Purification of polyclonal antibody (a week)
Polyclonal antibody purification(saturated ammonium sulphate ) |
Cat.No.:A4 |
¥500/20ml |
Polyclonal antibody purification(protein G) |
Cat.No.:A4 |
¥1000/20ml |
Polyclonal antibody purification(antigen specialized recognition) |
Cat.No.:A4 |
¥2000/1mg
Charged in protein yield |
Efficiency of protein G purification is about 14mg protein per 1ml antiserum
10mg antigen is required for purification of 20ml antibody by antigen affinity method.
Efficiency of protein G purification is about 14mg protein per 1ml antiserum
10mg antigen is required for purification of 20ml antibody by antigen affinity method.
Monoclonal antibody service (4-6 months)
Customer-tailored hybridoma service |
Cat.No.:A6 |
¥25000 |
To prevent the infection of contagious viruses and enhance immune response, we utilize SPF mouse with no pathogen. At the same time, for the best immune result, long period of immunization is required before cell fusion. As a result, it takes 4-6 months for hybridoma production. Aiming at reducing customer risks, we divide hybridoma production service into two stages, which is paid respectively.
Stage 1: immunization price: ¥3000
- Experiment design;
- Animal: five 6-8 week-old BALB/C mice;
- Antigen provided by customers should be more than 7mg and above 90% purity, which is verified in sds-page. If the project fails, the responsibilities are on customer’s shoulders if the antigen provided cannot meet the standard.
- Immunization goes for several times, titers are measured each time by ELISA.
- Verification method: ELISA test
- Verification standard: Antigen is specially recognized by the antiserum. Titer of antiserum is above 1:10,000
- After customer confirmation, we goes into step 2, or else the contract is terminated.
stage 2: establishment of antibody-excreting hybridoma? price: ¥22000
- Fusion of immunized spleen cell with myeloma. After selective cloning, we ultimately provide 2-3 cloned hybridoma and their corresponding hybridoma supernatant, or ascites in limited amount. Report of hybridoma establishment is offered( including fusion, cloning, antibody titer and subtype of the antibody)
- Verification method: ELISA test
- Verification standard: Antigen is specially recognized by the ascites or supernatant. And we only guarantee specialized recognition of antigens provided by the customer.
- Final provision: 2-3 hybridoma clones and corresponding ascites or supernatant, with their verification report(including subtypes)
Decomposition of monoclonal antibody service
To meet customer need and enhance agility of our services, we dismantle the whole monoclonal antibody production process into several separate services. Prices of each service are listed below:
Service name |
Service content |
Cat. No. |
price |
Mouse immunization |
Simultaneously immunize 5 BALB/C mice, and ELISA test |
A7 |
¥5000 |
fusion |
Fusion of spleen cell from one mouse with myeloma. Fusion rate and positive rate is calculated |
A8 |
¥3000 |
Mass fusion |
1-50 clones |
A9 |
¥1600 |
Clone proliferation and pre-selection |
Proliferation of a successful fusion product and stored at -20℃ |
A10 |
¥800 |
Subtype determination |
Verify the subtype of an antibody |
A11 |
¥150 |
subclone |
Select one positive clone |
A12 |
¥2250 |
Ascites production |
Ascites induction +ascites verification |
A13 |
¥3000 |
Antibody purification |
Protein G purification(IgG1、IgG2a、IgG2b、IgG3). Price is determined by the yield. Antibody must be preserved in special solution(i.e. PBS, pH 7.4). asepsis selection |
A14 |
>100mg¥1600
250mg¥2100
500mg¥3400
1g¥5700
2g¥9000
5g¥17000
10g¥34000 |
Western Blot verification |
Western blot service |
A15 |
¥1500 |
Antibody verification and label service
?
In content construction1`````` |
|
| Copyright
© 2005-2009 Antibody Research Center. All right reserved. |
|